【摘要】 本文采用三波长分光光度法同时测定食品中日落黄和柠檬黄。选择 30 5、347、4 6 4nm为测定日落黄的波长组合 ,其回归方程为ΔΑ =0 0 0 30 93+0 0 135 7C ,r日 =0 9999,线性范围为 3～ 95 μg·mL-1;选择 345、4 4 4、5 0 8nm为测定柠檬黄的波长组合 ,其回归方程为ΔΑ =0 0 0 1392 +0 0 2 5 6 1C ,r柠 =0 9999,线性范围为 4～ 85 μg·mL-1。实验表明 ,本方法很好的消除了日落黄与柠檬黄之间的干扰 ,回收率达到 95 5 6 %～10 2 1% ,RSD日 =0 6 2 31% ,RSD柠 =0 70 89% ,结果令人满意更多还原

【Abstract】 A new method for simultaneously determining sunset yellow and tartrazine in food with three-wavelength spectrophoto-method has been developed.Wavelength set is (305、347、464nm)for determining sunset yellow, and the regression equation isΔA=0.003093+0.01357C,r s=0.9999,the linear range is 3～95μg·mL -1;wavelength set is (345,444,508nm) for determining tartrazine ,and the regression equation isΔA=0.001392+0.02561C, r t=0.9999,the linear range is 4～85 μg·mL -1. The experiment demonstrated that the method can effective eliminate the interferences of sunset yellow and tartrazine ,the recovery is 95.56～102.1%,RSD s=0.6321%,RSD t=0.7098%,the result is satisfactory.更多还原