【摘要】 目的 :探讨内皮素受体拮抗剂在肝硬变门静脉高压症大鼠降压中的作用。方法 :32只雄性SD大鼠 ,随机分成 4组 ,每组 8只。其中模型对照组、BQ 12 3组、BQ 788组制备CCl4诱导的肝硬变模型 ,后 2组于造模成功后每d 2次分别皮下注射BQ 12 3(12 .5 μg/kg)和BQ 788(15 μg/kg) ;正常对照组 (NL) ,普通饲养 ,不做任何处理。采用生理八导仪测定门静脉压力 (PVP) ;RT PCR测定大鼠肝组织ET 1mRNA表达水平。结果 :BQ 12 3组 ,BQ 788组的PVP及肝组织ET 1mRNA表达量均较模型对照组显著降低 (P均 <0 .0 5 ) ,与正常对照组比较差异无统计学意义 ;模型对照组、BQ 12 3组、BQ 788组PVP与其ET 1mRNA的表达水平明显相关 (r分别 0 .94 4 ,0 .837和 0 .85 5 ;P均 <0 .0 5 )。结论 :应用内皮素受体拮抗剂能显著降低肝硬变门静脉高压症大鼠的PVP。更多还原

【Abstract】 Aim: To evaluate the effects of endothelin receptor antagonists on portal hypertension in cirrhotic hypertensive rat. Methods:Portal hypertensive models were induced by intragastric administration of carbon tetrachloride. Hepatic ET-1-mRNA was measured by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). Results: Portal pressure and expression of hepatic ET-1 mRNA were both increased after liver cirrhosis induced by carbon tetrachloride.However, ET receptor antagonists(BQ-123 or BQ-788 ) significantly reduced portal pressure and hepatic ET-1 mRNA expression at the same level. There was a positive correlation between portal pressure and hepatic ET-1-mRNA level. Conclusion: ET receptor antagonists could significantly reduce portal pressure in vivo.更多还原