【摘要】 目的 :制备盐藻核基质 ,进而分离核基质结合区 (matrixattachmentregions)。方法 :采用体积分数 0 .5 %TritonX - 1 0 0破碎细胞 ,体积分数 1 5 %Percoll分离盐藻细胞核 ,二碘水杨酸锂 (lithiumdiiodosalicylate,LIS)抽提核蛋白 ,SDS -PAGE电泳分析蛋白质成分。结果 :分离出了高纯度的盐藻细胞核 ,电泳分析表明绝大部分核蛋白已被去除。结论 :体积分数 0 .5 %TritonX - 1 0 0能有效破碎盐藻细胞 ,体积分数 1 5 %Percoll可分离高纯度的盐藻细胞核 ,2 5mmol/LLIS抽提可除去盐藻绝大部分核蛋白获得核基质更多还原

【Abstract】 Aim: Through preparing nuclear matrix of Dunaliella salina, in order to isolate the matrix attachment regions further.Methods: The D.salina cells were disrupted with 0.5%TritonX-100 and purified by centrifugation using 15% Percoll.The nuclear proteins were removed using 25 mmol/L lithium diiodosalicylate and the component of protein was analyzed by SDS-PAGE. Results: The pure nuclei of D.salina were isolated, and most protein component of nuclear protein was removed. Conclusion: The matrix of D.salina may be prepared through 25mmol/L LIS extracting nuclear protein.更多还原