【摘要】 目的 :探讨SOCS的表达与沙眼衣原体抑制宿主免疫功能导致持续性感染之间的关系。方法 :应用RT PCR和Westernblotting检测沙眼衣原体感染和未感染Hela2 2 9细胞SOCS 1,3mRNA水平和蛋白质水平的表达 ;免疫荧光检测反义寡核苷酸阻断SOCS 1,3表达后对沙眼衣原体增殖的影响。结果 :沙眼衣原体感染Hela2 2 9细胞后可诱导SOCS 1,3的表达 ,阻断SOCS 1,3表达后沙眼衣原体增殖受到抑制。结论 :沙眼衣原体感染Hela2 2 9细胞后可诱导SOCS 1,3的表达 ,SOCS 1,3的表达是沙眼衣原体抑制宿主免疫功能 ,使得其在宿主体内持续存在的原因之一更多还原

【Abstract】 Objective To explore the relationship between SOCS, immune suppression and chlamydial persistence of Chlamydia trachomatis-infected cells. Methods After being infected with Chlamydia trachomatis, RT-PCR was used to examine the expression of SOCS-1,3 mRNA and Western blotting to examine the expression of SOCS-1,3 protein in Hela229 cells. Immunofluorescence was used to examine the effect of SOCS obstruction on the multiplication of Chlamydia trachomatis. Results Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3 both on mRNA level and on protein level. The multiplicity of Chlamydia trchomatis can be suppressed after the expression of SOCS-1,3 was obstructed. Conclusion Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3, which is one of the mechanisms that Chlamydia trachomatis can suppress host immune and result in persistence.更多还原