【摘要】 目的 :探讨中药复方益气解毒颗粒抑制鼻咽癌细胞裸鼠移植瘤生长的分子机制。方法 :将鼻咽癌细胞系HNE1接种至BABL/c裸小鼠 ,2 0只 ,每只 0 .5× 10 7个 ,待移植瘤生长至一定大小 (约 1cm× 1cm× 1cm) ,随机分为两组。中药组灌喂益气解毒颗粒药液 ,对照组灌喂生理盐水 ,30d后处死裸鼠 ,解剖获取肿瘤组织 ,测量瘤体体积大小 ,称量瘤体重量。取 10 0mg瘤体组织 ,常规提取总蛋白质 ,用固相 pH梯度双向凝胶电泳分离移植瘤组织的总蛋白质 ,银染显色 ,ImageMaster 2DElite 4 .0 1软件分析差异蛋白质点。随机选取表达差异点共 19个 ,4个点为只在中药组表达 ,4个点仅在对照中表达 ,余下 11个为表达相差 5倍以上的点 ,进行质谱鉴定和生物信息学分析。结果 :益气解毒颗粒药液能明显抑制鼻咽癌细胞裸鼠移植瘤的生长 ,灌喂中药组瘤体积和重量分别为 (0 .2 0 7± 0 .0 2 3)cm3和 (0 .132± 0 .0 2 1) g ,对照组为 (1.342± 0 .2 98)cm3 和 (1.0 17± 0 .0 15 ) g(P <0 .0 5 ) ;制率为 84 .5 8%。双向电泳图像分析蛋白质表达 ,益气解毒颗粒药物处理组平均蛋白质数为 5 6 7± 4 9,未处理组的平均蛋白质数为 6 79± 5 9;差异蛋白质有 2 4 3个 ,其中有 112个表达增强 ,有 131蛋白质表达下降 ;差异蛋白质的质谱鉴定和生物?更多还原

【Abstract】 Objective To investigate the inhibitory mechanism of Yiqijiedu granule on the implanted-tumor growth of nasopharyngeal carcinoma in nude mice. Methods Twenty nude mice were injected nasopharyngeal carcinoma cells (HNE1), with 5×10 6 cells for a nude mouse. Implanted-tumors grew for 20 d, whose volume reached 1 cm×1 cm×1 cm. These nude mice were divided into 2 groups:Yiqijiedu group and control group.The Yiqijiedu group was given Yiqijiedu granule, and the control was given normal saline for 30 d,and then were killed. The volume and weight of implanted-tumors were measured.A 100-mg tissue from implanted-tumors was used to extract total protein by current methods, in which the proteins were separated by two-dimension electrophoresis and stained by silver, and protein profiles of implanted-tumors were obtained. Different proteins in the profiles were analyzed by ImageMaster 2D Elite 4.01. Nineteen different proteins, in which 4 expressed in the Yiqijiedu group, 4 expressed in the control, and the other 11 were differently expressed at 5 folds, were identified by MALDI-TOF-MS. Results The Yiqijiedu granule could inhibit the growth of implanted-tumors. The volume and weight of implanted-tumors in the Yiqijiedu group were (0.207±0.023)cm 3 and (0.132±0.021)g respectively,and that of the control was (1.342±0.298)cm 3 and (1.017±0.015)g(P<0.05). The inhibitory rate was 84.58%. The analyses of two-dimension electrophoresis and ImageMaster 2D Elite 4.01 showed that there were 567±49 protein dots in the Yiqijiedu group and 679±59 in the control. We found 243 proteins were dys-regulated, of which 112 proteins were observed, up-regulated and the other 131 proteins were down-regulated. MALDI-TOF-MS and Database analysis showed that the high expression proteins were HKR2 protein,10 Phosphoribosyl pyrophosphate synthetase, TNFR superfamily member, and Apoptosis regulator. The lower expression proteins were Fibulin-3,Zinc finger protein 266,Carboxy terminus of HSP70-interacting protein, et al. Conclusion Yiqijiedu granule could inhibit the growth of nasopharyngeal carcinoma, which may be associated with those proteins regulated by itself.更多还原