【摘要】 目的 :探讨大鼠心肌缺血 再灌诱导的基因表达谱改变 ,为揭示心肌内源性保护的分子机制提供新的线索和思路。方法 :采用反复短时间结扎及松解左冠状动脉主支构建大鼠心肌缺血 再灌反应动物模型 ,运用含 4 0 97个大鼠基因点的cDNA芯片检测大鼠心肌缺血 再灌处理后不同时间点 (1,3,6 ,12 ,2 4h)的基因表达情况 ,并采用SOM算法将具有相似表达模式的基因进行聚类。结果 :短时间缺血后再灌 1,3,6 ,12 ,2 4h分别有 75 ,779,2 0 5 ,15 5 ,16 6个基因表达发生改变。具有相同表达模式的基因被聚为 12类。结论 :心肌缺血 再灌可以诱导心肌的基因表达谱发生改变 ,具有相似表达模式的基因可能具有相似的功能或相似的表达调控机制。更多还原

【Abstract】 Objective To analyze the pattern of gene expression programs during rat myocardial ischemia/ reperfusion at multiple time points.Methods Rat model of myocardial ischemia/reperfusion was established by repeating the occlusion and relaxation of left coronary artery. cDNA microarray was used to analyze the pattern of gene expression programs in rat myocardium at 1,3,6,12,24 hours after reperfusion. SOM cluster analysis was used to identify different cluster of genes in which each cluster had similar expression pattern.Results Altogether 75, 779, 205, 155, and 166 genes were differentially expressed at 1, 3, 6, 12, 24 hours after the reperfusion respectively. Clusters analysis identified 12 clusters of genes in which each cluster had similar expression pattern. Conclusion Analysis of gene expression pattern revealed sequential induction of subsets of genes that characterize each response.更多还原