节点文献
百合组培快繁技术研究
Studies on the Technique of Tissue Culture and Rapid Propagation of Lily
【摘要】 以食用百合鳞片作外植体 ,以MS为基本培养基 ,对其组培快繁技术进行了研究。结果表明 ,适宜的芽诱导培养基为MS + 1 .0mg/L 6-BA + 0 .1mg/LNAA + 1 .0mg/LKT ;适宜的快繁培养基为MS + 1 .0mg/L 6-BA + 0 .0 1mg/LNAA + 0 .5mg/LKT ;适宜的生根培养基为MS + 0 .1mg/LIBA ;芽诱导和快繁较适宜的条件为光照 1 6h/d、温度 ( 2 5± 1 )℃ ;在光照 1 2h/d、温度 ( 2 5± 1 )℃条件下较易生根
【Abstract】 Using the edible bulb of Lilum from xinyang as the explant,a method for rapid propagation of Lily was established,based on MS medium. Among 16 medium combinations with NAA,KT and 6-BA concentrations,the appropriate mediums for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L KT;The best optimum medium for differentiation and subculture was MS+1.0 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L KT.The best medium for rooting was MS+0.1 mg/L IBA.The appropriate calture condition for bud induce and rapid propagation was light period of 16 h and temp of (25±1) ℃.
- 【文献出处】 河南农业科学 ,Journal of Henan Agricultural Sciences , 编辑部邮箱 ,2004年05期
- 【分类号】S644.1
- 【被引频次】15
- 【下载频次】464