【摘要】 为了建立玛咖的快速繁殖体系,以玛咖无菌苗的子叶为外植体进行愈伤组织诱导及形态发生研究。结果表明,激素配比为0.5mgL^-16-BA+0.5mgL^-1NAA获得的愈伤组织诱导率最高,达57.1%;适宜的分化培养基为MS+4.0mgL^-16-BA+0.5mgL^-1NAA,发芽率达到50%-60%;适宜生根培养基为1/2MS+0.5mgL^-1IBA,生根率达66.7%;经过16周的时间建立了完整的玛咖微繁殖系统。更多还原

【Abstract】 The callus induction and plant regeneration system for an important plant, Lepedium meyenii Walp.,has been established. Calli were induced from cotyledons petioles of Lepedium meyenii Walp within 4 weeks in a modified MS medium supplemented with BA plus NAA. The highest percentage of callus formation (57.1%) was found on MS medium supplemented with 0.5 mg l-1 BA and 0.5 mg l-1 NAA. During subculture on the shoot formation medium, most of calli proliferated and 50%-60%formed shoots. About 66.7%of shoots formed into roots on 1/2 strength MS containing 0.5 mg l-1 IBA after 4 weeks in culture. Chromosome count confirmed the number of the regeneration Maca plantlet was the same as that of the native plant(8x=64). For regeneration of plantlets, from seedling via primary callus production,a four step process of organogenes is required about 16 weeks.更多还原