【摘要】 重组质粒pMDIFN α中的猪α 干扰素基因经双酶切,亚克隆到原核表达载体pET 28a,构建了重组表达质粒pETIFN α,经过SDS PAGE蛋白质电泳,测得表达蛋白的相对分子质量约为26500.表达蛋白的Western blotting分析显示:改进后的半干胶转印免疫印迹法灵敏度高、特异性强、重复性好;获得的重组融合蛋白具有良好的反应原性.更多还原

【Abstract】 The expression plasmid pETIFN-α was constructed successfully and the recombinant procine IFN-α was expressed as inclusion bodies in E. coli. Sodium decylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a unique band with almost equal intensity with apparent raletive molecular mass of (26 500). The results of western-blotting indicated that the modified technique was highly sensitive, specific with good reproducibility, and the recombinant protein had good immunogenicity. The findings of the present study should have important implications for further studies on the prevention and control of viral diseases in pigs.更多还原