【摘要】 进行了洛克沙胂对鲫鱼的急性毒性试验,通过急性暴露胁迫对抗氧化系统的影响进行了探讨.试验发现,洛克沙胂对鲫鱼的急性毒性较低,24h LC50为181.69 mg/L,48 b LC50和96 h LC50均为176.57 mg/L.30、45 mg/L(以 As计)洛克沙胂连续暴露5 d,鲫鱼脑和肝乳酸脱氢酶受到显著抑制(P<0.05)并呈剂量-效应关系.鱼脑和肝的丙二醛含量显著升高(P<0.05).鲫鱼脑、肝和鳃组织的超氧化物歧化酶活性在初期显著升高(P<0.05),但在试验后期随剂量增高而受到显著抑制(P<0.05).鱼脑谷胱甘肽-S-转移酶受到极显著的抑制(P<0.01).结果表明:洛克沙胂对鲫鱼巯基酶和抗氧化功能有明显抑制,鱼脑和肝脏氧化损伤最明显,呈现剂量-效应关系.更多还原

【Abstract】 Toxic effects of roxarsone were investigated when carp (Carassias auratus) was exposed to roxarsone solution of 30 and 45 mg/L for 2 and 5 days. Roxarsone showed low toxicity to carp, but the margin of safety was narrow, the LC50 were as follows: 24 h LC50 181.69 mg/L, 48 and 96 h LC50 176.57 mg/L. The activities of LDH in carp brain and carp liver were inhibited significantly (P < 0.05) and showed dose-effect relationship. The contents of MDA in carp brain and carp liver increased significantly in all treated groups (P < 0.05) . Initially the activities of SOD in carp brain, liver and gill were enhanced significantly (P< 0.05) and then inhibited markedly with increasing doses. The activities of GSH-ST in carp brain were inhibited strikingly ( P <0.01) . These results showed roxarsone could inhibit enzyme with sulfhydryl group and the ability of antiox-idation. The oxidative damage to carp were remarkable and showed dose-effect relationship.更多还原