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犬精液液态保存稀释液及温度筛选试验

Selection of extenders and temperatures in liquid dog semen preservation

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【作者】 李彩霞魏庆信郑新民崔岘魏雁陈永昌

【Author】 Li Caixia 1 ,Wei Qingxin 2 ,Zheng Xinming 2 ,Cui Xian 1 ,Wei Yan 2 ,Chen Yongchang(1.Faculty of Animal Science And Technology,Gansu Agricultural University,Lanzhou730070;2.Bio-technology Institute of Hubei Academy of Agricultural Science,Wuhan,430072,China)

【机构】 甘肃农业大学动物科学技术学院湖北省农业科学院生物技术研究所甘肃农业大学动物科学技术学院 兰州730070武汉430064兰州730070兰州730070

【摘要】 探讨了8种稀释液在5℃、10℃、15℃和20℃下保存犬精液的效果。结果表明:15℃为犬精液液态保存的较适温度,3号液为优等液,其中3号液在15℃精子的存活时间为105±10h,与对照液在15℃的保存效果差异极显著(p<0.01)。2、4、5号液是良等液,在15℃精子的存活时间是64±8h,与对照液在15℃的保存效果差异显著(p<0.05)。中等液为6号液,在15℃、20℃保存时与对照液没有统计学上的差异(p<0.05),在其他2种温度保存时不及对照液。7、8号液为差等液,在15℃保存时与对照液差异不显著(p<0.05),在其他3种温度时不及对照液。

【Abstract】 The effects of dog semen preserved in8extenders at5 ℃ ,10 ℃ ,15 ℃ and20 ℃ were tested,the result showed:the ideal temperature was15 ℃ .After dog semen was incubated in different extenders,the third extender got the best result,and it’s life span was105 ± 10h at15 ℃ ,there was a high significant difference between the third extender and the first one(p < 0.01).The second,the fourth and the fifth were better extenders,sperm life span in those extenders were64 ± 8h at15 ℃ ,there was a significant difference between the second,fourth,fifth and the first extender(p < 0.05).The average extender was the sixth one,there were no statistical difference in sperm life span at15 ℃ and20 ℃ between the sixth extender and the first one(p< 0.05),but which less better than the first one.The seventh and eighth were the poor extenders,there was no statistical difference in sperm life span at15 ℃ (p < 0.05),but it’s less better than the first one at other temperatures.

【关键词】 精液稀释液温度精子存活时间
【Key words】 dogsemenextendertemperature