【摘要】 在花期分别对无融合生殖甜菜M14品系和进行正常有性生殖的二倍体甜菜A2Y品系的花蕾进行取样,采用TRIzol试剂一步法,分别提取了它们的总RNA,并测定了总RNA的浓度及纯度。讨论去除RNA酶的方法、具体操作的注意事项和TRIzol试剂一步法与其它方法相比的优越性。该方法为进一步进行mRNA差异显示分析和抑制消减杂交研究提供了总RNA的来源,也为今后能在甜菜中寻找和克隆与无融合生殖相关的基因提供了可靠的保证。更多还原

【Abstract】 M14, the apomictic monosomic addition line of Beta vulgaris, was first obtained in the world in 1995. In florescence, the buds of the M14 and B. vulgaris are taken to extract their total RNA using TRIzol reagent. The density and purity are determined. The methods of eliminating the RNase, the notes of the test and the merits of TRIzol reagent comparing with other methods are discussed. The system of extracting total RNA from sugar beet buds has been built which can offer a stable RNA resource for mRNA differential display and suppression subtractive hybridization. It can be further used to find and clone apomictic genes or relative genes. The system can provide a foundation for further study of apomictic sugar beet.更多还原