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HPLC法测定锦灯笼口服液中木犀草素的含量
Content Determination of Luteolin in Physalis permviana Liquid by HPLC
【摘要】 目的:建立了高效液相色谱法测定锦灯笼口服液中木犀草素含量的方法。方法:HPLC法,采用 Kromasn C18柱为分析柱,流动相为甲醇:水(56:44),流速1.0mL/min,检测波长为349nm。结果:木犀草素在0.02~0.10μg 之间呈良好的线性关系(r=0.9999),平均加样回收率为99.13%,RSD=1.82%。结论“该实验方法简便、可靠、灵敏、易行,可作为该制剂的质控方法之一。
【Abstract】 Objective.To develop a HPLC method for determining luteolin Liquid.Methods: The determination was carried on HPLC using Kromasil C18 column,Methanol—water(56:44)as mobile phase with a flow rate 1.0mL/min and detection wavelength at 349nm.Results:The cali- bration curve showed good lirearity over the range of 0.02~0.10μg(r=0.9999),the average re- covery was 99.13%,RSD=1.82%.Conclusion:This method is simple,reproducible,sensi- tive,and can be one of the method to prove the quality of this medicine.
- 【文献出处】 黑龙江医药 ,Heilongjiang Medical Journal , 编辑部邮箱 ,2004年01期
- 【分类号】R286
- 【被引频次】17
- 【下载频次】185