【摘要】 从石油污染土壤中分离纯化到 2株能以菲为唯一碳源和能源的细菌GF2和GF3,初步鉴定均为假单胞菌属 (Pseudo monas)细菌 .根据细菌在烃 -水两相体系中的细胞数量研究了GF2和GF3菌株的表面疏水性及其在不同环境条件下的变化 ,同时对GF2和GF3在液体培养条件下降解菲的效能进行了研究 .结果表明 ,GF2和GF3表面疏水性随培养时间、温度及pH值的变化而变化 ,GF3的疏水性 ( 74 8% )大于GF2 ( 5 8 6 % ) .在 2 5℃下培养 7d ,GF2和GF3对菲 ( 2 5 0mg·L-1)的降解率分别达到 85 6 %和 10 0 % .更多还原

【Abstract】 Two bacterial strains that could utilize phenanthrene as sole carbon and energy sources were isolated from oil-contaminated soils. The two strains w e re identified as Pseudomonas based on their morphological and physio-bioch emica l characteristics. The surface hydrophobicity of the two strains and its affecti ng factors were studied based on the cell amount of bacteria in hydrocarbon-wat e r two-phase system. The degradation of phenanthrene by GF2 and GF3 were carried out in solution. The results showed that the hydrophobicity of the two strains c hanged with the cultivation time, temperature and pH, and the surface hydrophobi city of strain GF3 （74.8%） was higher than that of strain GF2 （58.6%）.The degr ad ation rate of phenanthrene (250?mg·L^-1) by the strain GF2 and GF3 was 85 .6% and 100% respectively under 25℃ for 7?d.更多还原