【摘要】 以鲫鱼 (Carassiusauratus)作为实验对象 ,经过 4 0d不同浓度铜 (Cu2 + )及其配合物 (Cu EDTA)的暴露后 ,运用SDS PAGE和WesternBlotting方法检测鱼脑组织内应激蛋白HSP70的诱导表达情况 .结果表明 :在实验浓度下 ,与对照组相比 ,Cu2 + 和Cu EDTA对鱼脑内HSP70有显著的诱导 (p <0 0 5 ) ,而将Cu2 + 和Cu EDTA实验组相对比 ,络合剂EDTA一定程度上改变了Cu的生物可利用性 ,从而降低了其毒性 .实验还发现 ,Cu2 + 浓度在国家渔业水质标准 0 0 1mg/L下时 ,鱼脑组织内HSP70已经有明显的表达 ,说明运用分子生物学指标要比传统的环境监测指标更敏感 ,具有对污染物早期预警的作用 .更多还原

【Abstract】 The effects of copper (Cu 2+ ) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied.After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days , the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain.The results showed that Cu 2+ and Cu-EDTA complex can induce the expression of HSP70 significantly.Comparing with the test groups exposed to Cu 2+,the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal.It was found that the fish exposed to the concentration of Cu 2+ which below the national fishery water quality standard of 0.01mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.更多还原