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鱼鳞中羟脯氨酸的测定
Determination of Hydroxyproline in Fish Scales
【摘要】 用比色法测定鱼鳞中的羟脯氨酸 ( Hyp) ,具有较好的稳定性和较高的回收率。鱼鳞经过脱钙处理 ,羟脯氨酸的保留率为 96.1 9% ,经过第一次提取胶原蛋白后 ,提取率达到了总胶原蛋白的 70 %以上 ,同样的条件下进行第二次提胶 ,二次胶原蛋白的提取率较低
【Abstract】 The Hydroxyproline (Hyp) content in the fish scales was determined by the photocolorimetric method in this paper. The determination method is stable with a high yielding rate. After the de-calcification of the fish scales, the retaining rate of Hyp is 96 19%. The extract yield of Hyp from fish scales is more than 70% of the total collagen in the first time. However, under the same condition, the extract yield in the second time is rather low.
【基金】 江苏省教育厅自然科学研究指导性计划项目 ( 0 3 KJD2 40 0 3 3 )
- 【文献出处】 淮海工学院学报(自然科学版) ,Journal of Huaihai Institute of Technology , 编辑部邮箱 ,2004年01期
- 【分类号】Q51
- 【被引频次】35
- 【下载频次】582