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乳链菌肽产生菌P-99的摇瓶发酵条件
Study of fermentation conditions of nisin yield strain Streptococcus lactis P-99
【摘要】 文章对天然生物防腐剂乳链菌肽产生菌——乳链球菌P-99的摇瓶细胞生长和发酵条件进行了研究,发现该菌株细胞的生长与发酵是同步的,最适种龄为8~18h,产乳链菌肽表现出初级代谢动力学特征;在M17培养基、30℃、起始pH6.5条件下发酵良好,通气量和Ca2+、Zn2+、Fe2+等金属离子对其细胞生长与乳链菌肽的合成无明显影响,但Mn2+对乳链菌肽的合成有促进作用,而Cu2+则有较强的抑制作用。
【Abstract】 Cell growth and fermentation conditions of yield strain Streptococcus lactis P-99 were studied. Results showed that nisin production was synchronous with cell growth. The optimal pH and temperature for cell growth and nisin production in M17 medium were 6.5 and 30 ℃, respectively. Nisin production was increased by Mn<sup>2+ and inhibited strongly by Cu<sup>2+.
- 【文献出处】 合肥工业大学学报(自然科学版) ,Journal of Hefei University of Technology(Natural Science) , 编辑部邮箱 ,2004年11期
- 【分类号】Q939.97
- 【被引频次】4
- 【下载频次】200