【摘要】 目的 :探讨载脂蛋白B1 0 0 (ApoB1 0 0 )的体外氧化状态与其基本性质变化之间的关系。方法 :采用多聚阴离子沉淀、短时密度梯度超速离心法得到纯化的低密度脂蛋白 (LDL) ,经有机试剂抽提脂质 ,由半制备型分子排阻型高效液相色谱进一步纯化得到ApoB1 0 0。 2 ,2’ 盐酸脒基丙烷 (AAPH)氧化ApoB1 0 0 ,经反相高效液相色谱分析其疏水性质。结果 :体外轻微氧化ApoB1 0 0不会导致其降解 ,但其疏水性会增强。结论 :AAPH氧化ApoB1 0 0所致的早期疏水性改变 ,可能与LDL被清道夫受体识别有关 ,从而在动脉粥样硬化的发生发展中起到一定作用更多还原

【Abstract】 Objective: To investigate the relationship between oxidation of ApoB100 and its hydrophobic changes. Methods: To isolate low density lipoprotein (LDL) by using polyanions precipitation and short time density gradient ultracentrifugation.Organic reagents were used to delipidate LDL and ApoB100 was further purified by semi preparative size exclusion high pressure liquid chromato graphy(HPLC). After incubat with AAPH,the oxidized ApoB100 was loaded on reverse phase HPLC to analyze its hydrophobic changes. Results: Mild oxidation in vitro did not lead to ApoB100 fragmentation, but could increase its hydrophobicity. Conclusion: Oxidation of ApoB100 by AAPH increases its hydrophobicity at early stage,suggesting that hydrophobic changes of ApoB100 induced by AAPH may relate to LDL recognition by scavenger receptor,which may be included in pathophysiology of atherosclerosis.更多还原