节点文献

地衣芽孢杆菌海水生化需氧量传感器研究

BOD Biosensor for Seawater Using Bacillus Licheni Formis

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 马莉崔建升王晓辉刘俊稚张洁

【Author】 MA Li, CUI Jian-sheng, WANG Xiao-hui,LIU Jun-zhi, ZHANG Jie (College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang Hebei 050018, China)

【机构】 河北科技大学环境科学与工程学院河北科技大学环境科学与工程学院 河北石家庄 050018河北石家庄 050018河北石家庄 050018

【摘要】 用经海水驯化的地衣芽孢杆菌作响应菌株,采用夹层膜法制作固定化微生物膜,以氧电极为换能器制成BOD生物传感器;以空白海水为底液,传感器在BOD质量浓度为0~27mg/L范围内线性相关系数r=0.99978,对BOD质量浓度为4.31mg/L的GGA溶液连续平行测定7次,相对标准偏差为2.56%。

【Abstract】 Based on oxygen electrode,a BOD biosensor for seawater has been fabricated with immobilized cells using double-layered membrane method. The biological sensing material is immobilized Bacillus licheniformis tamed in saline water. With blank seawater as substrate, characteristics of the biosensor are tested. The biosensor has a linear response of 0 to 27 mg/L BOD and the linearity is perfect(r=0.999 78). The standard deviation of continuous measurements (n=7) is 2.56%. The sensor shows stability within 40 days without any decrease in signal.