【摘要】 以白菜高抗TuMV白心株系91 112和高感TuMV桔红心株系T12 91为亲本建立的小孢子DH系作为图谱构建群体,以AFLP标记来构建白菜连锁图谱。通过对64对AFLP引物组合的筛选,利用20对引物得到了263个AFLP多态性位点。经Mapmaker/EXP3.0软件处理,构建了1张含255个标记位点,10个连锁群,覆盖长度为883.7cM的连锁图。255个AFLP位点中偏离孟德尔遗传的比率(P<0.05)为15 0%和27 5%(P<0.01)。更多还原

【Abstract】 A molecular genetic map for Chinese cabbage was constructed based on AFLPs （Amplified Fragment Length Polymorphism） markers using DNA samples extracted from a doubled haploid population obtained through microspore culture from the F₁ between two homozygous parents: 91-112 and T12-19. By screening 64 AFLP primer combinations, 263 polymorphic bands were obtained from 20 pairs of primers. These genetic markers covered 883.7 centMorgans （cM） and belonged to ten linkage groups by using Mapmaker 3.0 software analysis. Markers Deviated from Mendelian segregation ratios were observed to be 15.0% （P<0.05） and （27.5%） （P<0.01） respectively.更多还原