【摘要】 目的 研究大蒜素对人早幼白血病HL-60细胞端粒酶活性的影响。方法 不同浓度的大蒜素作用HL-60细胞后,MTT法测细胞的生长和增殖情况,TRAP—PCR—ELISA法研究细胞端粒酶活性的变化。结果 大蒜素能明显地抑制HL-60细胞的生长,且呈时间、浓度依赖性。不同浓度的大蒜素能下调HL-60细胞的端粒酶活性,且同样呈时间、浓度依赖性。结论 大蒜素可抑制HL-60细胞的端粒酶活性,可能是其诱导HL-60细胞凋亡的重要机制之一。更多还原

【Abstract】 Objective To investigate the effect on telemerase of HL-60 cells treated by allicin. Methods HL-60 cells were treated by allicin with different concentrations. Then the inhibiting effect and the change of telomerase activity in HL-60 cells were detected by MTT assay and TRAP-PCR-ELISA. Results The proliferation of HL-60 cells treated with allicin was significantly inhibited, and it depended on density and time. Telomerase activity of HL-60 cells treated with allicin in different concentrations was significantly inhibited, and the effect also depended density and time. Conclusions Allicin can inhibit the telomerase activity of HL-60 cells. It may be one of important regulatory mechanisms in the apoptosis of HL-60 cell.更多还原