【摘要】 为了获得低温下PSⅡ颗粒复合物色素分子间的能量传递信息 ,运用荧光激发谱及荧光发射谱对 2 73K温度时不同波长光激发下菠菜的PSⅡ颗粒复合物的荧光光谱特性进行了研究 ,分析得出荧光强度的大小与激发光的波长有关 ,在实验所采用的激发波长中 ,4 36nm的光激发产生的荧光强度最大 ,5 2 0nm的最小 在能量传递过程中Chla分子的荧光效率最高 ,Chlb分子次之 结合吸收谱分析还发现在PSⅡ颗粒复合物中至少存在四种具特征吸收的Chl分子 :Chla6 6 16 6 0 ,Chla/b6 726 6 9,6 70 ,Chla6 826 80 ,Chla6 906 87 对 4 36nm光激发所得到的荧光发射谱进一步经高斯解析后得到五个光谱组分 :6 6 1nm ,6 72nm ,6 82nm ,6 90nm ,730nm ,所占的比例分别为 4 .75 % ,9.89% ,4 3.18% ,2 2 .0 8% ,2 0 .10 %更多还原

【Abstract】 In order to understanding spectral information on energy transfer in PSⅡ. PSⅡ particle complex purified from spinach was investigated with steady-state fluorescence excitation and emission spectroscopy at 273 k.The data were analyzed by Gauss analysis. The results showed that the fluorescence intensity of PSⅡ was different in the case of various wavelength excitation, the fluorescence intensity was maximum when excited with 436 nm, while excited with 520 nm got the minimum. In the process of energy transfer, Chl a had the highest energy transfer efficiency, and Chl b was in the next place. There are at least several characteristic Chl molecules existed in PSⅡ particle complex, i.e. Chl a 661 660,Chl a/b 672 669,670,Chl a 682 680,Chl a 690 687 (Chl a/b e a:a represents the peak of absorption,e represents the peak of emission). Moreover, five components were obtained with Gauss-fitting in the case of 436 nm excitation,they were 661.46 nm, 671.87 nm,682.25 nm,690.25 nm,729.65 nm, and their proportions were 4.75%,9.89%,43.18%,22.08%,20.10%,respectively.更多还原