【摘要】 目的　阐明饲料中添加烟酸对染矽尘大鼠早期Ⅰ型、Ⅲ型胶原形成的影响。方法　采用气管暴露法建立矽肺动物模型 ,高效液相色谱法测定血浆烟酸含量 ,天狼红染色法结合偏振光显微镜观察Ⅰ型、Ⅲ型胶原的形成 ,图像分析系统对胶原定量分析。结果　烟酸组不同时点血浆中烟酸含量明显升高 ,与对照组和矽尘组相比 ,差异均有显著性。染矽尘后第 7天、第 2 8天 ,与对照组比较 ,矽尘组大鼠肺组织Ⅰ型胶原阳性区域面积百分比显著增加 ,而烟酸组Ⅰ型胶原阳性区域面积百分比则明显低于矽尘组。染矽尘后第 2 8天 ,Ⅲ型胶原阳性区域面积百分比显著高于对照组 ,而烟酸组Ⅲ型胶原阳性区域面积百分比则显著低于矽尘组。结论　烟酸通过抑制Ⅰ型、Ⅲ型胶原的形成 ,而有效地阻断矽尘诱导的大鼠肺纤维化更多还原

【Abstract】 Objective To evaluate the effects of niacin in diet on early formation of Ⅰ and Ⅲ collagen in the lung of silica exposed rat.Methods Animal models were established by direct tracheal instillation of silica into rat lungs through tracheostomy.Serum niacin concentrations were analyzed by HPLC.Paraffin embedded lung sections were stained with Sirius red.Polarized light microscopy and Image Pro Plus detect Ⅰ and Ⅲ collagen.Results The results showed that the concentrations of niacin in serum of the experimented group were significantly elevated compared with control and silica treated groups.The elevation was reduced significantly by niacin.28 days after silica instillation,the percentage of Ⅲ collagen positive area was elevated significantly,and the elevation was reduced by niacin.Conclusions It concludes that niacin treatment could effectively hinder the lung fibrosis induced by silica through inhibiting the formation of Ⅰ and Ⅲ collagen in rat lung.更多还原