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产L-乳酸米根霉PW352特性及低能离子注入诱变高产菌株研究

Characteristics of L-lactic acid producing strain Rhizopus oryzae PW352 and high productive mutant by low energy ions implantation

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【作者】 古绍彬葛春梅汪青宏张洁姚建铭潘仁瑞余增亮

【Author】 GU Shao-bin 1, GE Chun-mei 1, WANG Qing-hong 2, ZHANG Jie 2, YAO Jian-ming 1, PAN Ren-rui 1,2, YU Zeng-liang 1 (1 Key Laboratory of Ion Beam Bioengineering of Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031; 2.Laboratory of Bioengineering of Hefei Union University, Heifei 230022 )

【机构】 中国科学院等离子体物理研究所离子束生物工程重点实验室合肥联合大学生物工程教研室中国科学院等离子体物理研究所离子束生物工程重点实验室 合肥230031合肥230031合肥230022合肥230031

【摘要】 为了获得更适于工业生产的产乳酸菌株 ,以葡萄糖为碳源对PW35 2菌株特性进行了研究 ,并在此基础上采用离子束诱变方法 ,对米根霉PW 35 2进行改良 ,获得高产L(+) 乳酸菌株RE330 3,产酸能力达 131~ 136 g L ,最高可达 14 0g L ,糖转化率为 86 %~ 90 % ,产酸比PW 35 2提高 75 %。

【Abstract】 In order to obtain higher L-lactic acid yield industrial strain, the characteristics and fermentation conditions of Rhizopus oryzae PW352 using glucose as carbon source were studied. The original strain PW352 was mutated by means of N + ions implantation and a mutant strain RE3303 was obtained. Its lactic acid production ability was increased by 75% than that of the original. The concentration of L-lactic acid reached to 131~136g/L and the conversion rate of glucose was as high as 86%~90% under the condition of 150g/L glucose in fermentation medium.

【关键词】 离子注入L(+)-乳酸根霉
【Key words】 ion implantationL(+)-lactic acidRhizopus oryzae
【基金】 国家十五攻关项目 ( 2 0 0 1BA3 0 2B)
  • 【文献出处】 工业微生物 ,Industrial Microbiology , 编辑部邮箱 ,2004年01期
  • 【分类号】TS252
  • 【被引频次】56
  • 【下载频次】281
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