【摘要】 资源冷杉(Abies ziyuanensis)属松科(Pinaceae)冷杉属(Abies),是国家一级保护的濒危植物。利用RAPD分子标记对分布于广西资源县银竹老山和湖南省炎陵县大院的2个资源冷杉种群共54株进行了遗传多样性分析。用20个随机引物共扩增到126个位点,其中66个位点是多态性的,总的多态位点百分率为52.4％,在两个种群分别为33.6％和31。2％。Nei’s基因多样性为0.312,Shannon信息指数为0.475,两个种群的遗传分化系数Gst为0.455。基于Nei’s遗传距离进行了UPGMA聚类分析,结果两种群的样品彼此明显区分开来。分析结果表明资源冷杉种群内的遗传多样性较低,但两种群间的遗传分化明显。需要同时重视两分布地的资源冷杉的保护工作。更多还原

【Abstract】 Abies ziyuanensis is a member of Pinaceae. Genetic diversity was studied using random amplified polymorphic DNA(RAPD)in 54 individuals of A. ziyuanensis from two populations in Ziyuan County of Guan-gxi and Yanling County of Hunan Province. The twenty primers used in this study amplified 126 scorable RAPD loci. The result of POPGENE analysis shows that the percentage of polymorphic loci is 52. 4% totaly, 33. 6% and 31. 2% in the two populations respectively,while Nei’s gene diversity is 0. 312,and Shannon’s information index is 0. 475. The coefficient gene differentiation (Gst) between the two populations is 0. 455. These indicate that there is low level of genetic diversity within populations,but high level of genetic differentiation between the two populations. The result suggested that the two populations of A. ziyuanensis should be considered individually when efforts were made to protect.更多还原