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黄皮愈伤组织诱导及其对不同激素的响应
Callus induction and respond to different phytohormone of Clausena lansium
【摘要】 通过对黄皮不同外植体进行离体培养 ,初步获得了愈伤组织。结果表明 :腋芽、花芽容易诱导绿色愈伤组织 ,且愈伤组织较细密。叶柄的愈伤组织诱导率很低 ,时间长 ,生长缓慢。愈伤组织的增殖继代培养的初步研究表明 :花芽愈伤组织在继代培养基上形成白色、较松散的愈伤组织 ,质地较差 ;而腋芽及叶柄产生的愈伤组织生长极缓慢 ,难以继代。
【Abstract】 Different explants of Wampee(clausena lansium) were used to induce callus.The preliminary results showed that the explant axillary bud and flower bud could be induced green,tighten callus in short time while the callus obtained from the explant petiole had lower induction rate,longer induction time,and grew much slowly.Subculture result indicated that white,friable callus could be obtained by subcultured callus from the explant of flower bud .However,the callus induced from the explant axillary bud and petiole grew much slow and it was difficult to subculture them.
- 【文献出处】 广西植物 ,Guihaia , 编辑部邮箱 ,2004年02期
- 【分类号】Q945
- 【被引频次】9
- 【下载频次】177