【摘要】 目的 :探讨抗卵巢癌细胞膜抗体作为放射免疫治疗载体的可能性 ,及对靶细胞体外裸鼠体内杀伤的相对特异性。方法 :采用补体介导细胞毒性试验 (MTT法 )测定抗卵巢癌细胞膜抗体对靶、非靶细胞比毒性 ;建立 BAL B/ C- m u裸鼠荷人卵巢癌模型 ,用 SPECT显像及病理检查分析 1 31 I标记抗卵巢癌细胞膜抗体在荷人卵巢癌裸鼠的聚集性。结果 :抗卵巢癌细胞膜纯化抗体比抗血清对卵巢癌靶细胞显示了更强的细胞毒作用 ,并且都显示了浓度梯度依赖性。抗卵巢癌细胞膜抗体对靶、非靶细胞比毒性显示了相对特异性 ,对卵巢癌细胞杀伤率高于非靶细胞 (宫颈癌、肝癌、胃癌、O型白细胞 )。并且呈时间依赖关系。1 31 I标记抗卵巢癌细胞膜抗体对荷人卵巢癌裸鼠腹腔注射 3d后 ,肿瘤区即有明显聚集 ,一直持续至第 8天。注射后第 8天 ,行病理检查 ,肉眼见有局部坏死淤血区 ;镜下见癌区组织大片状坏死 ,坏死区可见淋巴细胞浸润。结论 :抗卵巢癌细胞膜抗体对体内、体外卵巢癌细胞有相对特异性靶向性 ,具有作为放射免疫治疗载体的能力更多还原

【Abstract】 Objective:To investigate the possibility of anti-ovarian-cancer cell membrane antibody that can be used as a radioimmunotherapy vector, and the relative specific of kill effect on targeted cells in vitro.Methods:Cytoyoxocity test mediated with complement(MTT test)is conducted to measure the titer of anti-ovarian-cancer cell membrane antibody and its cytotoxicity to target/non-target cancerous cell. The carcinoma of ovarian model in BALB/C-mu nude mice was constructed. SPECT and pathological measurer are used to detect the radioactivity concentration of 131 I-labeled anti-ovarian-cancer cell membrane antibody in nude mice bearing human ovarian carcinoma.Result:With complement, the purified anti-ovarian-cancer cell membrane antibody showed stronger cytotoxicity ( killing rate ) in high concentration compared to the serum of anti-ovarian-cancer cell membrane antibody and the killing power’s dependency upon the concentration gradient. The cytotoxicity of anti-ovarian-cancer cell membrane antibody to target/non-target cancerous cell indicated the relative specific. The kill activity of the anti-ovarian-cancer cell membrane antibody to ovarian carcinoma could increase more than cervical carcinoma, hepatocarcinoma, stomach carcinoma and ‘O’type person’s leucocyte and showed dependency upon time. 131 I-labeled anti-ovarian carcinoma cell membrane antibody had obvious radioactivity concentration in the tumor mass after injected them into peritoneal cavity of nude mice bearing human ovarian carcinoma for 3 days; and then continued to the 8th day. When 8 days after dose application, the carcinoma tissues appear focal necrosis and extravasated blood in naked eyes and showed big pieces of necrosis infiltrated lymphocytes infra-microscope.Conclusion:The above studies proved that anti-ovarian-cancer cell membrane antibody had specific and targeted in killing ovarian cancer cells to some extent in vivo and in vitro, which would be expected as a novel vector for radioimmunotherapy.更多还原