【摘要】 香石竹茎尖培养玻璃化现象是香石竹脱毒试管苗生产的一个主要障碍 .采用强光照 10 0 0 0 -2 0 0 0 0LX ,在培养中提高糖和琼脂的浓度 ,降低细胞分裂素的用量 ,对克服香石竹茎尖培养玻璃化有明显效果 ,但对在茎尖培养中玻璃苗率高达 90 %左右的一些红色系品种效果并不理想 ,其中机制有待进一步研究。青霉素对消除香石竹茎尖培养玻璃化现象无明显效果。更多还原

【Abstract】 Vitrification is the main obstacle incarnation shoot tip culture. The effective way to decrease vitrification is to increase the concentration of sucrose and agar in the medium,to use strong luminosity of 10000-20000 Lx,and to decrease the concentration of cell derision hormone. However,this way has little effect on some of red-colored species,which have a high vitrification frequency of about 90% in shoot tip culture. There is no evident that penicillin has an effect on the decrease of vitrification.更多还原