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K562细胞摄取光敏剂ZnPcS₂P₂的测定及药代动力学

Determination and Pharmacokinetics of Photosensitizer ZnPcS₂P₂ Uptaken by K562 Cells

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【Author】 XIE Bao gang 1, HUANG Hui fang 2, HUANG Jian dong 1* , CHEN Nai sheng 1, HUANG Jin ling 1 1. Department of Chemistry, Institute of Research on Functional Materials, Fuzhou University, Fuzhou 350002, China 2. Institute of Haematology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, China

【机构】福州大学化学化工学院；福州大学功能材料研究所；福建医科大学附属协和医院血液病研究所；福州大学功能材料研究所福建福州350002；福建福州350001；福建福州350002；福建福州350002；

【摘要】通过荧光分析方法,比较了20%SDS(Wt%),15%TritonX100(φ为体积百分比V/V)及DMF等不同溶剂对K562细胞内摄取的两亲性酞菁锌ZnPcS2P2提取效率,结果表明TritonX100具有较高的一次提取率。以15%TritonX100为提取剂,获得了K562细胞摄取ZnPcS2P2及ZnPcS2P2低密度脂蛋白(LDL)复合物的动力学曲线。结果显示,K562细胞对ZnPcS2P2LDL的最大摄取量是ZnPcS2P2的两倍,表明LDL是有效的靶向载体。更多还原

【Abstract】 The di sulfonated di phthalimidomethyl phthalocyanine zinc(ZnPcS 2P 2) was an amphiphilic photosensitizer for photodynamic therapy of cancer. According to fluorescence analytical method, extracting efficiencies of 2.0% SDS(Wt%), 1.5% Triton X 100( φ ) and DMF for ZnPcS 2P 2 in K562 cells were compared. The results showed that 1.5% Triton X 100 was the most efficient reagent for the first time extracting. Using 1.5% Triton X 100 as extracting reagent, the kinetic curves of cellular uptake of ZnPcS 2P 2 as well as LDL ZnPcS 2P 2 complex by K562 cells were determined.Compared with ZnPcS 2P 2, the maximum cellular uptake of LDL ZnPcS 2P 2 by K562 was doubled, which indicated that LDL was an effective targeting delivery vehicle for ZnPcS 2P 2.更多还原