【摘要】 报道了功能性ZnS纳米荧光探针的合成与表征 ,同时研究了纳米粒子的荧光衰变寿命 ,考察了该纳米荧光探针的吸收光谱和荧光发射光谱特性。与此同时 ,研究了不同蛋白质和核酸对该纳米荧光探针的吸收光谱和荧光发射光谱的影响 ,实验表明 ,蛋白质的加入使得该纳米荧光探针的吸收和发射光谱强度均增强 ,而核酸的加入则使其吸收和发射光谱强度均减弱。据此 ,可望将该纳米荧光探针应用于生物大分子的分析测定更多还原

【Abstract】 In this work, nano-ZnS has been successfully prepared. The nano-ZnS has also been modified with sodium mercaptoacetic acid. The functionalized nanoparticles are water-soluble and biocompatible. All the nanoparticles have been characterized by IR spectra, UV spectra, fluorescence spectra and TEM images. In comparison with single organic fluorophore, the nanoparticles probes are brighter and more photostable, and do not suffer from blinking. The nanoparticles have a narrow, tunable, symmetric emission spectrum and a broad, continuous excitation spectrum. They are also photochemically stable. Effects of proteins and nucleic acids on the UV spectra and fluorescence spectra of the functionalized ZnS have also been studied. The intensities of UV spectra and fluorescence spectra of the functionalized ZnS are enhanced by proteins, and, however, are quenched by nucleic acids. The functionalized colloidal solutions prepared are hopeful of use as fluorescence probes in biological staining and diagnostics.更多还原