【摘要】 研究了基于聚合酶链式反应(PCR)检测饮用水中大肠杆菌的方法,所设计的引物对埃希氏大肠杆菌有特异性。该方法比较适于检测经氯剂消毒或臭氧消毒的饮用水中的大肠杆菌,检测结果与常规滤膜培养法有较好的一致性,且分析时间短(4～5h)、反应灵敏。更多还原

【Abstract】 The study was made on the method of polymerase chain reaction for detecting coliform in drinking water.The primer designed has specificity to E.coli..The method is more suitable for detecting the coliform in chlorine and ozone disinfected drinking water,and is in good agreement with the conventional membrane filter method.It is characterized by short period of analysis (4-5 h) and more sensitive detection.更多还原