【摘要】 用紫外 -可见光谱法研究了阳离子染料灿烂绿 (Brilliant Green)与脱氧核糖核酸 (fs DNA、ct DNA)相互作用的特性及分析应用。在 p H=8.0— 10 .5条件下 ,6 2 5 nm处的最大吸收峰随着 DNA量的增加 ,吸光度显著下降 ,并表现出一定的线性关系。据此 ,建立了一种可用于 DNA定量测定的新方法。其线性范围分别为 0 .10— 8mg/ L (fs DNA)、0 .15— 7mg/ L (ct DNA) ,表观摩尔吸光系数分别为 :6 .5 2× 10 4、9.0 8× 10 4L· mol-1· cm-1,相关系数分别为 0 .9988、0 .9990。方法具有较高的灵敏度和选择性。用于合成试样分析 ,结果令人满意更多还原

【Abstract】 The interaction of DNA(fsDNA,ctDNA) with Brilliant Green was studied by UV-visible spectrophotometry.In pH8-10.5 medium,the absorption at 625nm decreases linearly with the increase of DNA.A novel method for the determination of DNA at 625nm was developed in the linear range of 0.10-8mg/L for fsDNA and 0.15-7mg/L ctDNA with the correlation coefficients of 0.9988 and 0.9990,respectively.The method is sensitive and selective,and was used to determine DNA in synthetic samples with satisfactory results.更多还原