【摘要】 目的 :调查HIV - 1感染相关等位基因SDF - 3’A在我国畲族人群中的频率和多态性分布。方法 :随机采集血样 1 86份 ,提取基因组DNA。经PCR -RFLP分析 ,计算基因突变频率 ;并对群体分布和性别分布进行统计分析。结果 :SDF - 3’A频率为 1 9.6 %,群体分布符合Hardy -Weinberg平衡 ,性别之间无差异。结论 :畲族人的SDF- 3’A基因突变频率接近于新疆维吾尔族人。由于这种突变可延缓艾滋病的发病进程 ,这一发现对于畲族人的艾滋病防治具有积极意义。更多还原

【Abstract】 Objective:Genotyping and polymorphism of HIV-1 associated SDF1-3’A allele were investigated in She ethnic group.Methods:The whole blood samples from 186 She ethnic subjects were collected randomly,and their genomic DNA samples were extracted.Allelic polymorphism was identified by the PCR-RFLP analysis.The statistical significance of the data was further analyzed by χ 2 test.Results:The frequency of SDF1-3’A allele in She ethnic group sample was 19.6%.The allelic distribution of the gene was in accordance with Hardy-Weinberg equilirium.No difference existed between male and female individuals.Conclusion:The frequency of SDF1-3’A allele in She ethnic group is similar to that of Uigurian people.Based on its slow-down effect on clinical course of AIDS,the mutation of SDF1-3’A is highly significant in prevention and treatment of AIDS in She ethnic group.更多还原