【摘要】 从新疆荒漠地区分离到一株嗜热芽孢杆菌 ,该菌生长温度范围为 2 5～ 5 7℃ ,最适生长温度为 4 5℃ ,能产生耐热中性蛋白酶。经测定 ,酶的最适反应温度为 6 5℃ ,5 5℃处理 1h酶活无任何损失。酶的作用pH范围为 5 .0～ 9.0 ,pH稳定范围 7.0～ 8.5。野生菌在 1 1 0r/min ,4 5℃下培养 6 0h ,发酵液产酶为 5 0 0 0单位 /mL。更多还原

【Abstract】 The air temperature is high (the maximum air temperature can be up to 47.6 ℃, and the ground temperature can be over 70 ℃) in the Gurbantonggut Desert, Turpan Basin and desert regions in Karamay, Xinjiang, the soils are seriously salinized of alkalinized (in the Aydingkol lake region the Cl - content can be up to 35%, and the pH value of soil can be over 9.0), therefore, the fine conditions are provided for the existence salt tolerant and hot tolerant microorganisms. The thermophilic bacillus isolated from a desert area in Xinjiang can grow at a temperature range of 25～57 ℃, and the optimum temperature for its growth is 45 ℃. It can produce thermoduric neutral proteinase. The optimum catalytic temperature of the proteinase is 65 ℃. The activation of the proteinase under the temperature of 55 ℃ for one hour is 100%. The pH ranges of catalytic action and stability of the proteinase vary from 5.0～9.0 and from 7.0～8.5 respectively. Under the cultivating conditions on a shaking apparatus with a rotation speed of 110 r/min and temperature of 45 ℃ for 60 hours, the production of the proteinase is about 5000 μ/mL.更多还原