【摘要】 目的 :探讨胆管癌组织中 CD44m RNA表达与胆管癌转移的关系。方法 :采用 RT- PCR及 Southern杂交技术检测 2 6例胆管癌及 1 0例胆管良性病变标本中 CD44m RNA表达。结果 :1 6例胆管癌组织均能扩增出异常的 CD44v转录子片段 (>482 bp)。而 1 0例胆管良性病变组织只扩增出标准型的 CD44s转录子片段 (482 bp)。有淋巴结转移的胆管癌组织中出现较多异常的 CD44转录子片段。结论 :转录子数量较多的变异型 CD44v基因表达可能与胆管癌的转移扩散行为有一定的关系。更多还原

【Abstract】 Objective: To study the relationship between CD44 mRNA expression and tumor metastasis in cholangiocarcinoma. Methods: CD44 mRNA expression in 26 cholangiocarcinomas and 10 benign lesions of biliary duct was detected by RT-PCR and Southern blot. Results: Standard (482 bp, CD44s) and abnormal (>482 bp, CD44v) transcript fragments of CD44 were respectively amplified in 10 benign and 16 malignant lesions. CD44v expression was common in malignant lesions with nodal metastasis. Conclusion: Overexpression of CD44v could be related to the spread and metastasis of cholangiocarcinoma.更多还原