【摘要】 目的 :了解不同临床分期、组织学分级子宫颈鳞癌组织中端粒酶活性。方法 :采用以 PCR为基础的端粒酶重复扩增方法及非变性 PAGE银染定性法检测不同临床分期、组织学分级子宫颈鳞癌组织中端粒酶活性。结果 :在子宫颈癌组织中端粒酶活性的表达率较正常宫颈、不典型增生宫颈组织的端粒酶活性高 (P<0 .0 1 ) ,且不同临床分期的子宫颈癌宫颈组织间端粒酶的表达率差异有显著性 (P〈0 .0 5) ,但不同病理分级的子宫颈癌宫颈组织间端粒酶活性的表达率差异无显著性 (P>0 .0 5)。结论 :端粒酶在子宫颈癌组织中呈现高表达 ,且与临床分期有关 ,端粒酶可望成为检测子宫颈癌的一个重要指标。更多还原

【Abstract】 Objective: To observe the telomerase activity in squamous cell carcinoma of uterine cervix with different clinical stages and histological grades. Methods: Telomerase activity in squamous cell carcinoma, atypical hyperplasia, and normal specimen of uterine cervix was detected by PCR. Results: Telomerase activity in cervical carcinoma was significantly higher than that in atypical hyperplasia and normal tissue (P<0.01). In squamous cell carcinoma of uterine cervix, there existed statistical differences in telomerase activity among clinical stages (P<0.05) except among histological grades (P>05). Conclusion: Telomerase is overexpressed in squamous cell carcinoma of uterine cervix, and its expression is related to the clinical staging. Detection of telomerase activity is useful in judging the clinical stages of cervical squamous carcinoma.更多还原