【摘要】 目的 :观察碱性成纤维细胞生长因子 (b FGF)对成年家兔体外培养的视网膜组织的一氧化氮 (NO)及其合成酶 (NOS)的影响 ,旨在为视网膜移植提供抗氧化的保护方法。方法 :健康成年家兔 2 0只 (40眼 ) ,分成 4组 , (新鲜视网膜组 )、 (常规培养液组 )、 (高浓度b FGF液组 )、 (低浓度 b FGF液组 ) ,将 ～ 组视网膜组织块放入相应培养基中培养 1周。 4组视网膜取材后进行 NO、NOS生化指标的测定。结果 : ～ 组视网膜组织 NO、NOS含量差异有显著性 ,与 组相比 , 、 组 NO、NOS值显著增高 ;与 组相比 , 、 组 NO、NOS值显著降低 ; 组与 组 NO值统计学差异无显著性意义。 组与 组比较 ,NOS值降低明显。结论 :b FGF有清除自由基、增强抗氧化能力的作用 ,并且高浓度 b FGF较低浓度 b FGF对体外培养的视网膜组织抗氧化作用显著。更多还原

【Abstract】 Objective: To study the effect of basic fibroblast growth factor (bFGF) on nitric oxide (NO) and nitric oxide synthase (NOS) in rabbit retina. Methods: Twenty healthy adult rabbits (40 eyes) were randomly divided into 4 groups: Group Ⅰ (fresh retinal tissue), Group Ⅱ (routine culture), Group Ⅲ(high concentrations of bFGF), and Group Ⅳ (low concentrations of bFGF). The retinal tissue in each group other than Group I was cultured for 1 week NO,NOS levels in the retina were detected in all groups. Results: NO,NOS levels in Group Ⅱ were superior to those in other groups. There was no statistical difference in NO content between Group I and Group Ⅲ. NOS levels were significantly decreased in Group Ⅲ, compared with Group Ⅳ. Conclusion: bFGF is able to eliminate free radicals, and enhance the ability of anti-oxidation in retina, especially at high concentration.更多还原