【摘要】 目的 :探讨胆囊结石患者缩胆囊素 (CCK)受体基因表达及其与胆囊排空功能的关系。方法 :术前用B超测定 10 0例胆囊结石患者和 5 0例胃癌无胆囊结石患者的胆囊排空功能。胆囊壁CCK受体mRNA表达的测定用RT PCR法。结果 :胆囊结石组CCK受体mRNA表达 ( 0 .5 9± 0 .11)明显低于对照组 ( 0 .91± 0 .0 6 )(P <0 .0 1) ,该基因表达的降低与胆囊结石胆囊排空功能损害有关。胆囊结石患者的胆囊平滑肌细胞膜CCK受体mRNA表达与胆囊残余指数 (RV/FV× 10 0 % )呈负相关 (Y =0 .6 1- 0 .4 5X ,r =- 0 .6 5 ,P <0 .0 1)。结论 :胆囊结石患者CCK受体基因表达下调与胆囊排空功能的损害有关更多还原

【Abstract】 Objective:To investigate the expression of CCK receptor of gallbladder wall and the emptying function of the gallbladder in patients with gallstones.Methods:The gallbladder emptying function was determined preoperatively with B ultrasonographe and CCK receptor mRNA expression of gallbladder were studied with RT-PCR in 100 patients with gallstones and 50 patients of control group.Results:The gallbladder motility was significantly impaired in patients with gallstones. mRNA expression of CCK receptor of these patients was lower than that of control subjects(0.59±0.03 vs 0.91±0.04,P<0.01). Expression of CCK receptor mRNA in patients with gallstones(0.59±0.11) was decreased significantly than that of the control group. The change of expression of CCK receptors in the cellular membrane of the smooth muscle of the gallbladder negatively correlated with cholecystic remaining exponent(RV/FV×100%)(Y=0.61-0.45X,r=-0.65,P<0.01).Conclusion:The down-regulation of gene expression is related to the dysfunction of the cholecystic emptying function.更多还原