节点文献
含核酸碱基混合脂质体的制备与膜内分子识别
Preparation of Phosphatidylcholine/Cationic Amphiphilic Coliposomes and Molecular Recognition in the Bilayer Membranes
【Abstract】 An amphiphile, 9-octanyl adenine trimethylammonium bromide(OATMAB amphiphile 3), was synthesized as the receptor. The coliposomes were prepared from phosphatidylcholine and 9-octyl adenine trimethylammonium bromide by sonication of CHCl 3-cast films of amphiphiles mixtures [n(PC)∶ n(MOATMAB)=10∶1] in doubly distilled water with a bath-type sonicator at 80 W for 3 h. As thymine was added into PC/MAOTMAB coliposomal solution, the absorbability of thymine at 263 nm decreased slowly with time change from 0 to 72 h. The UV-Vis spectroscopic behavior of the thymine in PC/OATMAB/thymine coliposomes(molar ratio=10∶1∶1) was slowly decreaced with the increase of time. The onset of a hypochromic effect can be ascribed to adenine-thymine interaction. The absorption band levels off at 45.4% of its initial value and stabilizes after several weeks.
【Key words】 Nucleobases; Molecular recognition; Amphiphile; Coliposomes;
- 【文献出处】 高等学校化学学报 ,Chemical Research In Chinese Universities , 编辑部邮箱 ,2004年12期
- 【分类号】O629
- 【被引频次】2
- 【下载频次】136