【摘要】 采用时间分辨荧光光谱等手段研究了手性探针分子 L-[4-( 1 -芘基 ) ]丁酰基苯丙氨酸 ( PLP)与溶菌酶( Lys)、牛血清白蛋白 ( BSA)和人血清白蛋白 ( HSA)的结合过程及机理 ,探讨了三氯六氨合钴 ( Co HA)对不同复合体系内芘基的荧光猝灭作用 .结果表明 ,PLP在与血清白蛋白的结合过程中是以芘基插入到血清白蛋白疏水空腔中的方式与蛋白质结合的 ;而 PLP与 Lys的结合部位处在其表面的疏水部分更多还原

【Abstract】 Time-resolved fluorescence spectra was applied to studying the binding of L-[4-(1-pyrene)butyroyl]-phenylalanine(PLP) with lysozyme(lys), bovine serum albumin(BSA) and human serum albumin(HSA). The fluorescence lifetimes of pyrenyl group quenched by using cobalt(Ⅲ) hexaamine(CoHA) in free state and in different complexes protein systems were also investigated. These results suggest that PLP binds with SAs via its pyrenyl group insertion in the hydrophobic cavity of protein, whereas it binds with Lys in the hydrophobic area on the surface.更多还原