【摘要】 以玻碳电极为基础电极 ,用微波等离子体技术聚合沉积聚乙二胺等离子体膜 ,使之形成带氨基功能团的表面 ,再通过戊二醛交联共价固定辣根过氧化物酶 ,制得 H2 O2 生物传感器 .探讨了等离子体聚合膜的形成条件 (如放电功率、单体流速、单体气压和聚合时间 ) ,讨论了工作电位、介体浓度和 p H值对传感器响应的影响 .此外 ,用红外光谱对等离子体聚合膜进行了表征 .该传感器在 5× 1 0 - 7～ 1 .1× 1 0 - 3mol/L H2 O2浓度范围内有线性响应 ,最低检测限为 0 .3μmol/L.将此传感器用于实际试样回收率的测定 ,结果良好更多还原

【Abstract】 A biosensor for hydrogen peroxide assay based on glassy-carbon electrode with plasma polymerized polyethylene diamine film was proposed. Plasma polymerization technique was utilized to make amino groups deposit on the surface of glassy-carbon electrode for an oriented covalent attachment of HRP. The conditions of plasma polymerization including applied power for polymerization, pressure, plasma exposure time and flow rates were studied. The effects of experimental variables such as the operating potential of the working electrode, mediator concentration and pH of the measured solution were investigated for the optimum analytical performance by using an amperometric method, and the plasma polymerized polyethylene diamine film was examined by Fourier transform infrared spectroscopy. The biosensor provided a linear response to hydrogen peroxide over a concentration range of 5×10 -7—1.1×10 -3 mol/L and a detection limit of 0.3 μmol/L. The recovery studies for H 2O 2 in the real samples gave satisfactory results.更多还原