【摘要】 报道了在 CTAB/正己烷和 AOT/正己烷反胶束体系中 ,CCL脂肪酶催化合成异丁酸异戊酯的新方法 .考察了水含量 w0 、底物与酶的比例、缓冲溶液 p H值以及温度等因素对脂肪酶催化酯合成反应的影响 .研究结果表明 ,两种反胶束体系均为合成异丁酸异戊酯提供了较为合适的微环境 ,所选定的脂肪酶在CTAB和 AOT反胶束中的活性分别是有机溶剂中反应活性的 6倍和 4倍 .更多还原

【Abstract】 A new method of lipase-catalyzed esterification of isoamyl isobutyrate by CCL in the reversed micelle of CTAB/n-hexane and AOT/n-hexane has been reported. The effects of such factors as water content(w 0), ratio of substrate to enzyme, pH of buffer and temperature on the synthesis of esterification were investigated. Experimental results show that the reaction rate of esterification in the system of CTAB/n-hexane is faster than that in the system of AOT/n-hexane, and that the conversion of esterification reaches a climax of 99% in the two systems in 80 h. Both the two kinds of reversed micelle provide a suitable micro-environment for the selected lipase(CCL), which shows superactivity 6 and 4 times as much as that in organic solvent, respectively.更多还原