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OAP-H₂O₂-HRP酶促反应产物与DNA相互作用的光谱及电化学研究

Spectroscopic and Electrochemical Studies on the Interaction Between DNAand the Product of the Enzyme-catalyzed Reaction of OAP-H₂O₂-HRP

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【Author】 ZHANG Shu-Sheng 1, NIU Shu-Yan 1,2 , MA Li-Bo 1, JIAO Kui 1* (1. College of Chemistry and Molecular Engineering , Qingdao University of Science and Technology,Qingdao 266042, China; 2. College of Chemistry and Chemical Engineering,Ocean University of China, Qingdao 266003, China)

【机构】青岛科技大学化学与分子工程学院；青岛科技大学化学与分子工程学院青岛266042；青岛266042；中国海洋大学化学与化工学院；青岛266003；青岛266042；

【Abstract】 The pure product of 2-aminophenoxazine-3-one was prepared by enzyme-catalyzed reaction and analyzed. The electrochemical behaviour of 2-aminophenoxazine-3-one on a glass carbon electrode was investigated. The interaction between DNA and 2-aminophenoxazine-3-one was investigated by cyclic voltammetry and UV-Vis spectroscopy. The peak potential of 2-aminophenoxazine-3-one in pH 6.8 phosphate buffer solution in the presence of DNA shifts positively, indicating that the bind of 2-aminophenoxazine-3-one with DNA is intercalation action. The influence of reacting time was also studied. The binding ratio of the complex of DNA and 2-aminophenoxazine-3-one at room temperature is calculated to be 1∶1, and the binding constant is to be 6.60×10 4 L/mol.更多还原