【摘要】 用酶法在受控条件下降解玉米醇溶蛋白 ,对酶解产物进行分离纯化 ,获得一种新的抗氧化肽 ;对其一级结构进行了表征 ,氨基酸组成和顺序为 Leu-Asp-Tyr-Glu;从结构上分析了其可能的抗氧化机制更多还原

【Abstract】 A kind of new corn anti oxidative peptide was prepared via protease hydrolysis of zein catalyzed by alcalase, an alkaline protease under a certain condition, followed by isolation and purification. The hydrolysis time was fixed on the basis of the dependence of antioxidative activity on hydrolysis degree. It was found that the hydrolysis for 4 h gave the highest antioxidative activity. The isolation and purification steps include ultrafiltration with a membrane of cutoff M w 1 000, successive chromatography on Sephadex G 15, Amberlite CG 120(Na) ion exchange, Sephadex G 10 and preparative HPLC columns. The amino acid sequence of the antioxidative peptide was characterized to be Leu Asp Tyr Glu by amino acid analysis and electrospray ion trap mass spectrometry. We also discussed the possible anti oxidative mechanism of the antioxidative peptide on the basis of its structure.更多还原