【摘要】 根据抑癌基因 ING1基因的序列设计并合成了检测 ING1转录产物的分子信标核酸探针 ,发展了一种快速测量从正常细胞系和鼻咽癌肿瘤细胞系提取的 ING1转录产物的方法 ,所得结果与用逆转录结合 PCR法 ( RT-PCR)得到的结果相吻合 .并且 ,将能表达 ING1基因的质粒转入肿瘤细胞进行培养后 ,再将分子信标转入肿瘤细胞 ,发现转导了质粒的肿瘤细胞比未转导质粒的肿瘤细胞内的荧光明显增强 ,从而进一步证实了所设计的分子信标核酸探针与 ING1转录产物的结合更多还原

【Abstract】 Three molecular beacons(MBs) were synthesized to find a suitable probe to hybridize the expression of ING1 gene, a recently identified tumor suppressor gene. The loop sequences of MB1 and MB2 are the anti sense and sense sequence of ING1, respectively. The sequence of MB3 is a piece of ssRNA sequence in tobacco mosaic virus, which has no analogy to human gene. MB1 is the most suitable probe because MB1 has the highest fluorescence enhancement after hybridizing with RNA extracted from normal cell. A simple method has been built for the quantitative analysis of ING1 mRNA from nasopharyngral carcinoma(NPC) cell lines and normal cells. The results show that the concentration of ING1 mRNA in normal cells is higher than that in tumor cells. This result is consistent to that of RT PCR detection. ING1 plasmid was delivered into living human NPC cells with liposome and the expression of ING1 by plasmid was monitored by the selected MB.更多还原