【摘要】 以 AT切型、基频 10 MHz的镀金膜石英晶体作为换能器 ,将抗人胰岛素和 C肽单克隆抗体固定在石英晶体电极表面 ,用 2× 5检测池固定夹具构建一种新型压电胰岛素 - C肽微阵列免疫传感器 .研究了抗体固定方法、抗体工作浓度、固定量、一致性以及传感器的响应参数如检测温度、时间和特异性等的影响 .该微阵列传感器在胰岛素浓度为 2 .5～ 16 0 .0 m IU/ L、C肽浓度为 0 .375～ 12 .0 ng/ m L范围内响应特性良好 ,压电晶体频率偏移值与胰岛素和 C肽浓度呈良好的线性关系 .将此微阵列传感器用于人血清标本的测定 ,结果与放射免疫法符合 ( r为 0 .92和 0 .94 ) .此微阵列传感器具有灵敏度高、特异性好 ,低密度阵列结构 ,检测通量较高 ,不需标记 ,操作简单、能实时在线检测和重复使用等优点 ,能用于临床实验诊断 ,具有临床推广应用价值 .更多还原

【Abstract】 A new 2×5 type of piezoelectric insulin-C peptide micro-array immunosensor was developed for the determination of insulin and C peptide. The energy converters were 10 MHz AT-cut quartz crystals with gold-coated electrodes. The monoclonal antibodies of insulin and C peptide were immobilized onto the surfaces of crystals gold electrodes respectively, then piezoelectric insulin-C peptide micro-array immunosensor was constructed by 2×5 type of immobilization clamping apparatus. The effects of methods, work concentrations, the amount and coherence of immobilization of antibody and the response parameters of the micro-array immunosensor such as temperature, time-cost and specificity etc. were investigated. Under the optimized conditions, the experimental results showed piezoelectric immunosensor had good response to insulin and C peptide whose frequency shifts were linearly dependent on insulin concentration in the range of 2.5—160.0 mIU/L and C peptide concentration in the range of 0.375—12.0 ng/mL. The piezoelectric micro-array immunosensor were used to detect insulin and C peptide in serum, the analytical results given by this approach were in satisfactory agreement with those given by radiometric immunoassay(P>0 05), with correlation coefficients 0 92 and 0 94. The piezoelectric micro-array immunosensor for the determination of insulin and C peptide is of high sensitivity, high specificity, low density micro-array structure, high analysis speed, unnecessary labelling, simple operation, real-time detection and repeated use, etc.. It is suitable for detecting insulin and C peptide of serum in clinical laboratory.更多还原