【摘要】 目的　探讨蛋氨酸合成酶 (MS)基因A2 75 6G突变在阿尔茨海默病 (AD)发病中的意义。方法　PCR扩增 6 6例AD患者及 14 3例对照者的MS基因突变点 ,经限制性内切酶消化后行凝胶电泳确定其基因型。结果　MS基因片段PCR扩增产物长度分别为 189bp。MSA2 75 6G突变的G等位基因PCR扩增产物经HaeⅢ消化后裂解成 15 9和 30bp两个片段 ;MAD组A2 75 6G基因型频率 (% )分别为 98 4 8、1 5 2和 0 0 0 ,对照组分别为 98 6 0、1 4 0和 0 0 0 ,MSA 2 75 6G各种基因型频率在患者组与正常对照组之间的差异无显著性 (P >0 0 5 )。结论　MS多态性与AD无明显相关。更多还原

【Abstract】 Objective To explore the significance of gene mutations of methione synthase(MS) in patients with Alzheimer desease(AD), and analyse the relation between them. Method The genotyes of MS were determined by PCR-based assay in 66 patients with Alzheimer desease and 143 healthy controls.Results There were no siganificant differences in the frequenceies of MS mutations between the patients and contral groups(P>0.05).Conclusion MS may not be independent risk factors for Alzheimer′s disease.更多还原