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碱提猴头发酵菌丝体水溶性多糖HPP的结构确定

Structure studies on the water soluble polysaccharide HPP isolated by alkali from the fermentative mycelia of Hericium erinaceus

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【作者】 耿慧梁忠岩赵伟张雅君

【Author】 GENG Hui~1,LIANG Zhong-yan~1,ZHAO Wei~(1,2),ZHANG Ya-jun~1 (1.School of Life Sciences Northeast Normal University,Changchun 130024,China; 2.College of Air Force Flight in Changchun,Changchun 1300222,China)

【机构】 东北师范大学生命科学学院东北师范大学生命科学学院 吉林长春130024吉林长春130024吉林长春130024空军长春飞行学院吉林长春130022吉林长春130024

【摘要】 从猴头发酵菌丝体水提后残渣中用碱提出了水溶性粗多糖,对其分级纯化得到纯样HPP,SepharoseCL-6B柱层析测得其相对分子质量约为6 53×104,G C 分析表明,其单糖组成为Glc;经部分酸水解、高碘酸氧化、Smith降解和甲基化分析确定此多糖为多分枝结构,其中(1→6)Glc构成主链的核心结构;分支点为O-3;支链部分由(1→3)Glc构成,并连接一端基Glc.

【Abstract】 A water soluble crude polysaccharide has been isolated by alkali from the water-boiled fermentative mycelia of hericium erinaceus pers.After graded and purified,HPP was obtained,whose molecular weight was about 65.3 thousand by gel filtration on sepharose (CL-6B,)and it was glucan by G.C.analysis.Furthermore,by analyzing the products of partial hydrolysis with acid,periodate oxidation,smith degradation and methylation,the structure of HPP was determined as (1→)-linked-Glc Composing of the backbone,(1→3)-linked-Glc residues were substituted at O-3,and its side chain was made up of(1→3)-linked-Glc,as well as one deoxidized Glc.

【关键词】 猴头菌多糖结构
【Key words】 hericium erinaceuspolysaccharidestructure
【基金】 国家自然科学基金资助项目(39870847)
  • 【文献出处】 分子科学学报 ,Journal of Molecular Science , 编辑部邮箱 ,2004年02期
  • 【分类号】R284
  • 【被引频次】25
  • 【下载频次】200
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