【摘要】 探讨超氧化物歧化酶(SOD)对电离辐射和肿瘤化疗药物对荷瘤机体正常组织损伤的保护效应及机制,测定荷瘤鼠肝脏、脾脏、肾脏、心脏、肺、脑和骨髓组织的活性氧(ROS)、氧化产物丙二醛(MDA)水平和谷胱苷肽过氧化物酶(GSH?Px)、过氧化氢酶(CAT)活性,并观察SOD对荷瘤小鼠肝脏超微结构的影响。结果表明,荷瘤小鼠受照射和化疗药物后其骨髓、肝脏、肾脏、脾脏、组织匀浆中ROS、MDA含量明显升高,而GSH?Px和CAT活性明显降低,其机制是电离辐射和化疗药物造成抗氧化酶的损伤及机体内ROS含量上升。加入SOD与对照组相比,显著降低荷瘤小鼠骨髓、肝脏、脾脏组织匀浆中ROS、MDA含量,略微升高GSH?Px和CAT活性,而对肾脏组织匀浆中ROS、MDA含量和GSH?Px和CAT活性无明显影响,同时SOD可明显减轻电离辐射对荷瘤小鼠肝细胞超微结构破坏,SOD通过直接清除自由基和保护抗氧化酶的损伤起辐射保护作用。更多还原

【Abstract】 In order to investigate protection effects of SOD on the oxidized damage of normal tissues induced in chemotherapeutic drug and irradiated tumor?bearing mice, the contents of ROS and MDA, the activation of GSH?Px and CAT in liver, kidney, spleen and bone marrow have been determined. The effect of SOD on the ultra?structure of liver cell has been evaluated. It has been found that chemotherapeutic drug and irradiation can increase the contents of ROS and MDA and decrease activation of GSH?Px and CAT. And SOD can decrease contents of ROS and MDA and increase the activation of GSH?Px and CAT slightly for liver, spleen, marrow, but for kidney there is no significant change while SOD can reduce the damage of ultra?structure of liver. It can be inferred that SOD have some protec- tion effects on the normal tissue of tumor?bearing mice via scavenging free radicals and activate anti?oxidative en- zymes after TBI and drugs injection.更多还原