【摘要】 在pH1.0～2.3的酸性介质中,水溶性苯胺蓝与牛血清蛋白、人血清蛋白、卵白蛋白、α 糜蛋白酶作用形成结合产物时将导致溶液光吸收发生变化,在558nm褪色,在634nm附近吸光度增强,且吸光度差(△A)值均与蛋白浓度成正比,不同蛋白质分别在0～50mg/L或20～80mg/L范围内符合比耳定律。据此,建立了一种新的光度测定蛋白质的新方法。它用于人血清和尿液样品中总蛋白质的质量测定,回收率在95%～102%之间,结果满意。更多还原

【Abstract】 In pH 1.0～2.3 acidic medium, water-soluble aniline blue reacts rapidly with proteins to form complexes, which causes the change of absorption spectra, the decrease of absorbance near 558 nm and the increase of absorbance near 634 nm. All △A（=A-A₀） values are proportional to the concentration of proteins. Beer’s law is obeyed in the range 0～50 mg/L or 20～80 mg/L for the different proteins. Their molar absorptivities of fading reactions are between 1.72×10⁵ and 1.99×10⁶ L·mol^-1·cm^-1. Based on these, we developed a new spectrophotometric method for the determination of proteins. The method is simple, rapid, repeatable and selective, and has been used for the determination of total amounts of proteins in human serum and urine samples with satisfactory results.更多还原